PHF1 anticorps (Internal Region)

N° du produit ABIN6264170

Cet anticorps Lapin Polyclonal détecte spécifiquement PHF1 dans WB, ELISA, IF et ICC. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour PHF1 anticorps (Internal Region) (ABIN6264170)

Antigène: PHF1 (PHD Finger Protein 1 (PHF1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PHF1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-PHF1 anticorps

Épitope: Internal Region

Specificité: PHF1 Antibody detects endogenous levels of total PHF1.

Homologie: Pig,Horse,Sheep,Rabbit

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human PHF1, corresponding to a region within the internal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur PHF1

Antigène: PHF1 (PHD Finger Protein 1 (PHF1))

Autre désignation: PHF1

Sujet:

Description: Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci (PubMed:18285464 and PubMed:23273982). According to another report, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2 (PubMed:23142980). Regulates p53/TP53 stability and prolonges its turnover: may act by specifically binding to a methylated from of p53/TP53.

Gene: PHF1

Poids moléculaire: 62kDa

ID gène: 5252

UniProt: O43189