PKM anticorps (Internal Region)

N° du produit ABIN6264260

Cet anticorps Lapin Polyclonal détecte spécifiquement PKM dans ELISA, WB, IHC, IF et ICC. Il présente une réactivité envers Humain, Souris et Rat et a été mentionné dans 1 publication.

Aperçu rapide pour PKM anticorps (Internal Region) (ABIN6264260)

Antigène: PKM (Pyruvate Kinase M1/2 (PKM))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PKM est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-PKM anticorps

Épitope: Internal Region

Specificité: PKM2 Antibody detects endogenous levels of total PKM2.

Homologie: Pig,Bovine,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human PKM2, corresponding to a region within the internal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Références pour anticorps anti-PKM (ABIN6264260)

Xie, Huang, Tang, Ye, Yang, Guo, Tian, Xie, Peng, Xie: "Diallyl Disulfide Inhibits Breast Cancer Stem Cell Progression and Glucose Metabolism by Targeting CD44/PKM2/AMPK Signaling." dans: Current cancer drug targets, Vol. 18, Issue 6, pp. 592-599, (2018) (PubMed).

Détails sur PKM

Antigène: PKM (Pyruvate Kinase M1/2 (PKM))

Autre désignation: PKM

Sujet:

Description: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Gene: PKM

Poids moléculaire: 58 kDa

ID gène: 5315

UniProt: P14618

Pathways: L'effet Warburg