RPA2 anticorps (N-Term)

N° du produit ABIN6264708

L’anticorps Lapin Polyclonal anti-RPA2 a été validé pour WB, ELISA, IHC, IF et ICC. Il convient pour détecter RPA2 dans des échantillons de Humain et Souris.

Aperçu rapide pour RPA2 anticorps (N-Term) (ABIN6264708)

Antigène: RPA2 (Replication Protein A2, 32kDa (RPA2))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RPA2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-RPA2 anticorps

Épitope: N-Term

Specificité: RPA32/RPA2 Antibody detects endogenous levels of total RPA32/RPA2.

Homologie: Pig,Zebrafish,Bovine,Sheep,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human RPA32/RPA2, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur RPA2

Antigène: RPA2 (Replication Protein A2, 32kDa (RPA2))

Autre désignation: RPA2

Sujet:

Description: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.

Gene: RPA2

Poids moléculaire: 32kDa

ID gène: 6118

UniProt: P15927

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA