TOP1MT anticorps (Internal Region)

N° du produit ABIN6265672

Cet anticorps anti-TOP1MT est un anticorps Lapin Polyclonal détectant TOP1MT dans WB et ELISA. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour TOP1MT anticorps (Internal Region) (ABIN6265672)

Antigène: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TOP1MT est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-TOP1MT anticorps

Épitope: Internal Region

Specificité: TOP1MT Antibody detects endogenous levels of total TOP1MT.

Homologie: Pig,Zebrafish,Horse,Sheep,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human TOP1MT, corresponding to a region within the internal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur TOP1MT

Antigène: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Autre désignation: TOP1MT

Sujet:

Description: Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).

Gene: TOP1MT

Poids moléculaire: 70 kDa

ID gène: 116447

UniProt: Q969P6