FBXO5 anticorps (N-Term)

N° du produit ABIN6295468

Cet anticorps Souris Monoclonal détecte spécifiquement FBXO5 dans WB, IF et IHC. Il présente une réactivité envers Humain.

Aperçu rapide pour FBXO5 anticorps (N-Term) (ABIN6295468)

Antigène: FBXO5 (F-Box Protein 5 (FBXO5))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FBXO5 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Détail du produit anti-FBXO5 anticorps

Épitope: N-Term

Fonction: Mouse anti-Human EMI-1 Antibody [Sodium Azide Free]

Specificité: Nuclear

Purification: A human partial recombinant N-terminal protein fragment was used as the immunogen for the EMI-1 antibody.

Immunogène: A human partial recombinant N-terminal protein fragment was used as the immunogen for the EMI-1 antibody.

Information d'application

Indications d'application: Western blot: 1-2 μg/mL
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Buffer: In 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Détails sur FBXO5

Antigène: FBXO5 (F-Box Protein 5 (FBXO5))

Autre désignation: F-box only protein 5

Sujet:

Target Description: Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI-1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of EMI-1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Gene Symbol: FBXO5///EMI1///FBX5

ID gène: 26271

UniProt: Q9UKT4

Pathways: Mitotic G1-G1/S Phases