CD34 anticorps

N° du produit ABIN6295964

L’anticorps anti-CD34 Monoclonal Souris est utilisé pour la détection de CD34 dans des échantillons de Humain, Cynomolgus et Macaque rhésus. Il a été validé pour FACS, IHC et IF.

Aperçu rapide pour CD34 anticorps (ABIN6295964)

Antigène: CD34

Reactivité: Humain, Cynomolgus, Macaque rhésus

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD34 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-CD34 anticorps

Fonction: Mouse anti-Human/Cynomolgus Monkey/Rhesus Monkey CD34 Antibody [Sodium Azide Free]

Specificité: Cell surface

Purification: A detergent solubilized vesicular suspension prepared from human term placenta was used as the immunogen for this CD34 antibody.

Immunogène: A detergent solubilized vesicular suspension prepared from human term placenta was used as the immunogen for this CD34 antibody.

Information d'application

Indications d'application: FACS: use antibody at 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Détails sur CD34

Antigène: CD34

Autre désignation: Hematopoietic progenitor cell antigen CD34

Sujet:

Target Description: This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. This is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. CD34 antibody labels > 85 % of angiosarcoma and Kaposi 's sarcoma, but with a lower specificity.

Gene Symbol: CD34

ID gène: 947

UniProt: P28906