Keratin 77 anticorps

N° du produit ABIN6296409

Cet anticorps anti-Keratin 77 est un anticorps Souris Monoclonal détectant Keratin 77 dans IHC, WB, IF et FACS. Adapté pour Humain, Rat, Boeuf (Vache), Chien, Souris, Lapin, Poulet et Singe.

Aperçu rapide pour Keratin 77 anticorps (ABIN6296409)

Antigène: Keratin 77 (KRT77)

Reactivité: Humain, Rat, Boeuf (Vache), Chien, Souris, Lapin, Poulet, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Keratin 77 est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-Keratin 77 anticorps

Fonction: Mouse anti-Human/Monkey/Cow/Dog/Rabbit/Mouse/Rat/Chicken pan Cytokeratin Antibody Cocktail [Sodium Azide Free]

Specificité: Cytoplasmic

Purification: Human epidermal keratin was used as the immunogen for the pan Cytokeratin antibody cocktail.

Immunogène: Human epidermal keratin was used as the immunogen for the pan Cytokeratin antibody cocktail.

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Buffer: In 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Détails sur Keratin 77

Antigène: Keratin 77 (KRT77)

Autre désignation: Keratin, type II cytoskeletal 1b

Sujet:

Target Description: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 55 kDa (CK7), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 46 kDa (CK17), 45 kDa (CK18) and 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.

Gene Symbol: KRT77///KRT1B///KRT76///KRT2B///KRT2P

UniProt: Q7Z794, Q01546