Major Capsid Protein L1 anticorps

N° du produit ABIN6296477

Détail du produit

Antigène: Major Capsid Protein L1

Reactivité: Human Papillomavirus 16 (HPV-16)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Fonction: Mouse anti-Type 16 of Human Papilloma Virus (HPV-16) L1 Antibody [Sodium Azide Free]

Specificité: Nuclear

Purification: Human papilloma virus type 16, major capsid protein L1, was used as the immunogen for the L1 antibody.

Immunogène: Human papilloma virus type 16, major capsid protein L1, was used as the immunogen for the L1 antibody.

Information d'application

Indications d'application: Western blot: 1-2 μg/mL,ELISA: assay dependent
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Stockage

Buffer: In 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Détail du antigène

Antigène: Major Capsid Protein L1

Synonymes: anticorps major capsid protein L1, anticorps L1

Classe de substances: Viral Protein

Sujet:

Target Description: Reacts with a protein of 57 kDa, identified as the L1 protein of human papilloma virus type 16 (HPV-16). Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on the basement membrane to provide initial virion attachment to target cells. Basement membrane is exposed only after epithelium trauma. Additionally, the alpha6 integrin complexed with either beta1 or beta4 integrin has been proposed to act as a coreceptor recognized by L1. Once attached, integrin complexed with beta4 integrin has been proposed to act as a coreceptor recognized by L1. Once attached, the virion enters the host cell via clathrin-mediated endocytosis and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2. [UniProt]The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16 or -33, very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. It cross-reacts with HPV37.

Gene Symbol: L1

UniProt: P03101