Cyclin B1 anticorps

N° du produit ABIN6297159

Cet anticorps anti-Cyclin B1 est un anticorps Souris Monoclonal détectant Cyclin B1 dans IHC, IF et FACS. Adapté pour Humain et Souris.

Aperçu rapide pour Cyclin B1 anticorps (ABIN6297159)

Antigène: Cyclin B1 (CCNB1)

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin B1 est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-Cyclin B1 anticorps

Fonction: Mouse anti-Human/Mouse CCNB1 Antibody [Sodium Azide Free]

Specificité: Cytoplasmic & nuclear

Purification: Recombinant human full-length protein was used as the immunogen for the CCNB1 antibody.

Immunogène: Recombinant human full-length protein was used as the immunogen for the CCNB1 antibody.

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Buffer: In 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Détails sur Cyclin B1

Antigène: Cyclin B1 (CCNB1)

Autre désignation: G2/mitotic cyclin-B1

Sujet:

Target Description: It recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Gene Symbol: CCNB1///CCNB

ID gène: 891

UniProt: P14635

Pathways: Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase