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GAPDH anticorps (N-Term)

Cet anticorps anti-GAPDH est un anticorps Lapin Polyclonal détectant GAPDH dans WB. Adapté pour Humain et Chien. Ce Primary Antibody a été cité dans 2+ publications.
N° du produit ABIN630723

Aperçu rapide pour GAPDH anticorps (N-Term) (ABIN630723)

Antigène

Voir toutes GAPDH Anticorps
GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivité

  • 221
  • 153
  • 133
  • 65
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  • 19
  • 15
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  • 9
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  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Humain, Chien

Hôte

  • 167
  • 91
  • 16
  • 9
  • 4
Lapin

Clonalité

  • 185
  • 100
Polyclonal

Conjugué

  • 165
  • 31
  • 19
  • 16
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
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  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp GAPDH est non-conjugé

Application

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  • 77
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  • 41
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  • 36
  • 33
  • 16
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  • 15
  • 15
  • 7
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  • 5
  • 4
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB)
  • Épitope

    • 36
    • 23
    • 13
    • 11
    • 8
    • 7
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    Specificité

    GAPDH antibody was raised against the N terminal of GAPDH

    Purification

    Affinity purified

    Immunogène

    GAPDH antibody was raised using the N terminal of GAPDH corresponding to a region with amino acids IDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKW
  • Indications d'application

    WB: 1 µg/mL
    Optimal conditions should be determined by the investigator.

    Commentaires

    GAPDH Blocking Peptide, (ABIN939307), is also available for use as a blocking control in assays to test for specificity of this GAPDH antibody

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Lyophilized powder. Add distilled water for a 1 mg/mL concentration of GAPDH antibody in PBS

    Concentration

    Lot specific

    Buffer

    PBS

    Conseil sur la manipulation

    Avoid repeated freeze/thaw cycles.
    Dilute only prior to immediate use.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.
  • Kuroda, Ishii, Uematsu, Ohata, Coban, Akira, Aritake, Urade, Morimoto: "Silica crystals and aluminum salts regulate the production of prostaglandin in macrophages via NALP3 inflammasome-independent mechanisms." dans: Immunity, Vol. 34, Issue 4, pp. 514-26, (2011) (PubMed).

    Shi, Zhang, Yang, Zhang, Wei: "ROCK1 plays an essential role in the transition from cardiac hypertrophy to failure in mice." dans: Journal of molecular and cellular cardiology, Vol. 49, Issue 5, pp. 819-28, (2010) (PubMed).

  • Antigène

    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

    Autre désignation

    GAPDH

    Sujet

    GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.

    Poids moléculaire

    36 kDa (MW of target protein)
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