CD147 anticorps

N° du produit ABIN638433

Cet anticorps anti-CD147 est un anticorps Souris Monoclonal détectant CD147 dans WB, FACS et Func. Adapté pour Humain. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour CD147 anticorps (ABIN638433)

Antigène: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD147 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

Clone: MEM-M6-6

Détail du produit anti-CD147 anticorps

Fonction: Anti-Hu CD147 Purified Low Endotoxin

Specificité: The antibody MEM-M6/6 recognizes extracellular Ig domain D2 (membrane proximal) of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells, it is not expressed by resting lymphocytes.

Réactivité croisée (Details): Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

niveau d'endotoxine: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogène: Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimmer).

Isotype: IgG1

Information d'application

Indications d'application: Functional application: The antibody MEM-M6/6 (high-affinity mAb of unique epitope specificity) inhibits anti-CD3-induced T cell activation.
Flow cytometry: recommended dilution: 1-4 μg/mL.
Western blotting: Positive control: 293 human fibroblastoid cell line, non-reducing conditions.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD147 (ABIN638433)

Koch, Staffler, Hüttinger, Hilgert, Prager, Cerný, Steinlein, Majdic, Horejsí, Stockinger: "T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density." dans: International immunology, Vol. 11, Issue 5, pp. 777-86, (1999) (PubMed).

Détails sur CD147

Antigène: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Autre désignation: CD147

Sujet: Basigin (Ok blood group),CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN (extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derived collagenase-stimulatory factor) is an ubiquitously expressed cell surface protein with multiple glycosylated forms. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumour cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.,Neurothelin, Basigin, LAM6, CSF, 5F7, TCSF, BSG, TCSF, Emprin, OK blood group

ID gène: 682

UniProt: P35613

Pathways: S100 Proteins