Sonic Hedgehog anticorps (C-Term)

N° du produit ABIN6391361

Détail du produit anti-Sonic Hedgehog anticorps

Antigène: Sonic Hedgehog (SHH)

Épitope: C-Term

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Sonic Hedgehog est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Fonction: SHH

Séquence: DSEALHPLGM AVK

Réactivité croisée: Chien, Humain, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 48 kDa band observed in Human Liver lysates (calculated MW of 49.6 kDa according to NP_000184.1 Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Liver for t

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the membranes of HeLa, U251 and NIH3T3 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concen

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur Sonic Hedgehog

Antigène: Sonic Hedgehog (SHH)

Autre désignation: SHH (SHH Produits)

Synonymes: anticorps HHG1, anticorps HLP3, anticorps HPE3, anticorps MCOPCB5, anticorps SMMCI, anticorps TPT, anticorps TPTPS, anticorps 9530036O11Rik, anticorps Dsh, anticorps Hhg1, anticorps Hx, anticorps Hxl3, anticorps M100081, anticorps fc83d08, anticorps shh, anticorps syu, anticorps vhh-1, anticorps vhh1, anticorps wu:fc83d08, anticorps Xhh, anticorps hedgehog, anticorps xshh, anticorps SHH, anticorps twh, anticorps twhh, anticorps sonic hedgehog, anticorps sonic hedgehog a, anticorps sonic hedgehog L homeolog, anticorps sonic hedgehog protein A, anticorps sonic hedgehog b, anticorps SHH, anticorps Shh, anticorps shha, anticorps shh.L, anticorps shh, anticorps shhb

Sujet: SHH, sonic hedgehog homolog (Drosophila), HGNC:10848, HHG1, HLP3, HPE3, SMMCI , sonic hedgehog

ID gène: 6469

NCBI Accession: NP_000184

Pathways: Signalisation Hedgehog, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development