Complement C4d anticorps

N° du produit ABIN640791

Cet anticorps anti-Complement C4d Monoclonal Souris (ABIN640791) détecte spécifiquement Complement C4d dans ELISA, IF, WB, IHC (p) et IHC (fro). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Complement C4d anticorps (ABIN640791)

Antigène: Complement C4d (C4d) (Complement Component C4d (C4d))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Complement C4d est non-conjugé

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Complement C4d anticorps

Fonction: Mouse Anti-Human C4d

Specificité: DS-MB-00163 recognizes the secreted protein complement component 4d (C4d). The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b's alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonisation and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. C4d's short half life means that C4d is present in serum at high enough concentrations to make it a useful marker for classical complement activation. C4 has been linked to susceptibility to systemic lupus erythematosous. ImmunohistologyIt has been reported that this antibody works very well on acetone-fixed, frozen renal biopsies. Strong staining is observed in the glomeruli and in some cases the peritubular capillaries. DS-MB-00163 has given variable results on formalin-fixed, paraffin-embedded sections. It has been observed that pre-treatment with 88% formic acid for 20 minutes at room temperature is beneficial (6).

Attributs du produit: Blood Coagulation Proteins

Purification: Purified

Immunogène: Native, from human plasma

Isotype: IgG1

Information d'application

Indications d'application: Immunohistology - Frozen, ELISA (dilution: 1/250, 1/600), Immunofluorescence, Immunohistology - Paraffin, Western Blotting

Restrictions: For Research Use only

Stockage

Concentration: IgG concentration 1.0 mg/ml

Buffer: Borate buffered saline pH 8.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Détails sur Complement C4d

Antigène: Complement C4d (C4d) (Complement Component C4d (C4d))

Autre désignation: C4d