DFFA anticorps

N° du produit ABIN6566873

L’anticorps Lapin Polyclonal anti-DFFA a été validé pour WB et IF. Il convient pour détecter DFFA dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour DFFA anticorps (ABIN6566873)

Antigène: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DFFA est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-DFFA anticorps

Purification: Affinity purification

Immunogène: Recombinant protein of human DFFA

Isotype: IgG

Information d'application

Indications d'application: WB 1:500 - 1:2000 IF 1:50 - 1:100

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Buffer: PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze / thaw cycles.

Détails sur DFFA

Antigène: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Autre désignation: DFFA

Sujet:

Synonyms: A330085O09Rik,Caspase activated deoxyribonuclease inhibitor short form,DFF 1,DFF 45,DFF alpha,DFF-45,DFF1,DFF35,DFF45,DFFA,Dffa DNA fragmentation factor,alpha subunit,DFFA,DNA fragmentation factor 45 kDa subunit,DNA Fragmentation Factor Alpha Subunit,DNA fragmentation factor subunit alpha,DNA fragmentation factor,45 kD,alpha subunit,DNA fragmentation factor,45  kDa,alpha polypeptide (DFFA),transcript variant 1,DNA fragmentation factor,45  kDa,alpha polypeptide,DNA fragmentation factor,alpha subunit,DNAation factor 45 kDa subunit,H13,ICAD,ICAD L,ICAD S,Inhibitor of CAD,Inhibitor of Caspase Activated DNase,MGC143066,OTTHUMP00000001903,OTTHUMP00000001904,RP23 121D17.3

Background: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Poids moléculaire:

Observed_MW: 35kDa, 45kDa

Calculated_MW: 29kDa/36kDa

ID gène: 1676

UniProt: O00273

Pathways: Apoptose, Caspase Cascade in Apoptosis