Recombinant ALK anticorps (full length)

N° du produit ABIN6654206

Cet anticorps anti-ALK Monoclonal Lapin (Clone ATKR-2R) (ABIN6654206) détecte spécifiquement ALK dans IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Recombinant ALK anticorps (full length) (ABIN6654206)

Antigène: ALK (Anaplastic Lymphoma Receptor tyrosine Kinase (ALK))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp ALK est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: ATKR-2R

Détail du produit anti-ALK anticorps

Épitope: full length

Fonction: Recombinant ALK Antibody / Anaplastic Lymphoma Kinase

Purification: Protein A affinity

Immunogène: Recombinant full length human protein was used as the immunogen for this recombinant ALK antibody.

Isotype: IgG, kappa

Information d'application

Indications d'application: Optimal dilution of the recombinant ALK antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the recombinant ALK antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur ALK

Antigène: ALK (Anaplastic Lymphoma Receptor tyrosine Kinase (ALK))

Autre désignation: ALK

Sujet: Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK. [UniProt]

UniProt: Q9UM73

Pathways: Signalisation RTK