CD40 Ligand anticorps (AA 108-261)

N° du produit ABIN6654286

L’anticorps Souris Monoclonal anti-CD40 Ligand a été validé pour IHC (p). Il convient pour détecter CD40 Ligand dans des échantillons de Humain.

Aperçu rapide pour CD40 Ligand anticorps (AA 108-261) (ABIN6654286)

Antigène: CD40 Ligand (CD40LG)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD40 Ligand est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CD40LG-2761

Détail du produit anti-CD40 Ligand anticorps

Épitope: AA 108-261

Purification: Purified

Pureté: Protein G affinity chromatography

Immunogène: A portion of amino acids 108-261 from the human protein was used as the immunogen for the CD40 Ligand antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the CD40 Ligand antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. IHC (FFPE): 1-2 μg/mL for 30 minutes at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Stockage

Buffer: 0.2 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the CD40 Ligand antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur CD40 Ligand

Antigène: CD40 Ligand (CD40LG)

Autre désignation: CD40L / TRAP / CD154

Sujet: CD40LG expression is mainly confined to the CD4-positive-T-cell subset. Its expression is induced shortly after T-cell activation and represents an early activation marker of T lymphocytes. CD40 is constitutively expressed mainly on B cells, macrophages, and dendritic cells. The CD40-CD40L pathway has been shown to play multiple functional roles in the healthy immune system. It enhances the antigen-specific T-cell response through the activation of dendritic cells and the induction of interleukin-12 production. For example, engagement of CD40 on endothelial cells by activated T cells expressing CD40L leads to upregulation of adhesion molecules such as ICAM-1, VCAM-1, and E-selectin. Activation of APC by CD40-CD40L interaction induces the production of inflammatory cytokines, chemokines, NO, and metalloproteinases. Interaction of CD4-positiveCD40LG-positiveT cells with CD40 on B cells leads to B-cell differentiation, proliferation, immunoglobulin (Ig) isotype switching, and formation of memory B cells.

Pathways: Signalisation NF-kappaB, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints