Recombinant BrdU anticorps
Aperçu rapide pour Recombinant BrdU anticorps (ABIN6654472)
Antigène
Voir toutes BrdU AnticorpsType d'anticorp
Hôte
Clonalité
Conjugué
Application
Classe de qualité
Clone
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Fonction
- BrdU Antibody / Bromodeoxyuridine (azide and preservative free)
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Purification
- Protein A affinity chromatography
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Immunogène
- BrdU / Bromodeoxyuridine conjugated to KLH was used as the immunogen for this BrdU antibody.
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Isotype
- IgG
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Indications d'application
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine / BrdU antibody to be titered up or down for optimal performance.
1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1 mg/mL PBS, 10 min at 37oC. -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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Agent conservateur
- Azide free
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- BrdU (Bromodeoxyuridine (BrdU))
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Autre désignation
- BrdU
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Classe de substances
- Chemical
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Sujet
- This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine / BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Antigène
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