DMPO anticorps (FITC)

N° du produit ABIN6655017

N° du produit (Fournisseur): 200-302-f21

Cet anticorps Souris Monoclonal détecte spécifiquement dans IP, WB, IF, IHC et FM. Il présente une réactivité envers Adenovirus.

Aperçu rapide pour DMPO anticorps (FITC) (ABIN6655017)

Antigène: DMPO

Reactivité: Adenovirus

Hôte: Souris

Clonalité: Monoclonal

Conjugué: FITC

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Clone: N1664A

Détail du produit

N° du produit (Fournisseur): 200-302-f21

Fournisseur: Rockland

Fonction: DMPO Antibody

Réactivité croisée (Details): DMPO is species independent.

Purification: Anti-DMPO Antibody was purified by Protein G chromatography.

Stérilité: Sterile filtered

Immunogène: DMPO Antibody was produced in mice by repeated immunizations with 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry_Dilution: User Optimized

IF_Microscopy_Dilution: User Optimized

Commentaires:

Anti-DMPO Antibody is tested for use in WB, IP, IHC and IF microscopy. Expect a band approximately ~90kDa corresponding to specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M Tris Cl, 0.15 M Sodium Chloride, 0.001 M EDTA, pH 7.4

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.05 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détail du antigène

Antigène: DMPO

Classe de substances: Chemical

Sujet:

Synonyms: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline N oxide

Background: The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states. The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems. Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals. DMPO adducts can be generated with protein and DNA radicals.