Acetylated Lysine anticorps

N° du produit ABIN6655030

N° du produit (Fournisseur): 200-301-a97

L’anticorps anti-Acetylated Lysine Monoclonal Souris est utilisé pour la détection de Acetylated Lysine. Il a été validé pour WB, ELISA et IHC.

Aperçu rapide pour Acetylated Lysine anticorps (ABIN6655030)

Antigène: Acetylated Lysine

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Acetylated Lysine est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 7F8

Détail du produit anti-Acetylated Lysine anticorps

N° du produit (Fournisseur): 200-301-a97

Fournisseur: Rockland

Fonction: Lysine Acetylated Antibody

Réactivité croisée (Details): This antibody is known to react with acetylated lysine residues from mammalian and avian sources.

Purification: This Protein G purified monoclonal antibody reacts with proteins containing acetylated lysine residues and does not detect non-acetylated lysine residues.

Stérilité: Sterile filtered

Immunogène: This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with acetylated keyhole limpet hemocyanin (KLH).

Isotype: IgG1

Information d'application

Indications d'application:

ELISA_Dilution: User Optimized

Immunohistochemistry_Dilution: 1:200 - 1:1,000

Western_Blot_Dilution: 1:500 - 1:2,000

Other: User Optimized

Commentaires:

This Protein G purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Approximately 1 μg of antibody is sufficient to detect 20 μg of acetylated chicken erythrocyte histones (sodium butyrate treated) by Western blotting using HRP Gt-a-Mouse IgG (p/n 610-103-121) and chemiluminescent substrate (p/n PICOMAX-110). Antibody provided in PBS pH 7.4.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: See application note.

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Acetylated Lysine

Antigène: Acetylated Lysine

Autre désignation: Lysine Acetylated

Classe de substances: Chemical

Sujet:

Synonyms: acetyl Lysine antibody, Acetylated lysine antibody, Lysine antibody

Background: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. A common posttranscriptional modification of lysine residues involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4). HDACs therefore are promising targets for anticancer drugs currently in development (5).