Progesterone Receptor anticorps (AA 412-526)

N° du produit ABIN6655214

Détail du produit anti-Progesterone Receptor anticorps

Antigène: Progesterone Receptor (PGR)

Épitope: AA 412-526

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Progesterone Receptor est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purification: This product is clarified tissue culture supernate. The antibody is specific for human Progesterone Receptor protein. Cross-reactivity with Progesterone Receptor protein from other sources has not been determined.

Immunogène:

Immunogen: Anti-Progesterone Receptor monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 412-526 of human Progesterone Receptor protein. The hybridoma was produced by fusing New Zealand White rabbit splenocytes and myeloma cells using conventional technology.

Immunogen Type: Recombinant Protein

Clone: SP2

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:400

Application Note: This monoclonal antibody contains TBS/1 % BSA and is suitable for ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 116 kDa in size corresponding to PgR isoform B (the 'canonical' sequence) protein by western blotting in the appropriate cell lysate or extract. Progesterone Receptor is found in human Breast ductal carcinoma and is a nuclear protein. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Deparaffinize slides using xylene or xylene alternatives and graded alcohols. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min.

ELISA Dilution: 1:10,000

Western Blot Dilution: 1:200-1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.1 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date.

Références pour anticorps anti-Progesterone Receptor (ABIN6655214)

Huang, Zhu, Szekeres, Xia: "Development of new rabbit monoclonal antibody to estrogen receptor: immunohistochemical assessment on formalin-fixed, paraffin-embedded tissue sections." dans: Applied immunohistochemistry & molecular morphology : AIMM, Vol. 13, Issue 1, pp. 91-5, (2005) (PubMed).

Cano, Milanezi, Leitão, Ricardo, Brito, Schmitt: "Estimation of hormone receptor status in fine-needle aspirates and paraffin-embedded sections from breast cancer using the novel rabbit monoclonal antibodies SP1 and SP2." dans: Diagnostic cytopathology, Vol. 29, Issue 4, pp. 207-11, (2004) (PubMed).

Détails sur Progesterone Receptor

Antigène: Progesterone Receptor (PGR)

Autre désignation: Progesterone Receptor (PGR Produits)

Synonymes: anticorps NR3C3, anticorps PR, anticorps 9930019P03Rik, anticorps BB114106, anticorps ENSMUSG00000074510, anticorps PR-A, anticorps PR-B, anticorps XPR-1, anticorps pgr, anticorps xpr-2, anticorps LOC443444, anticorps PGR, anticorps pg, anticorps pr, anticorps gb:dq017620, anticorps progesterone receptor, anticorps progesterone receptor L homeolog, anticorps PGR, anticorps Pgr, anticorps pgr.L, anticorps pgr

Sujet:

Synonyms: rabbit anti-Progesterone Receptor Antibody, PgR, PR, nuclear receptor subfamily 3 group C member 3

Background: The PGR gene encodes a member of the steroid receptor superfamily referred to as progesterone receptor (also known as PgR, PR and nuclear receptor subfamily 3 group C member 3). Progesterone receptor mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform A only, and mediate their own response genes and physiologic effects with little overlap. Ideal for Cancer, Chromatin & Nuclear Signaling and Signal Transduction research.

Gene Name: PGR

ID gène: 5241

UniProt: P06401

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Smooth Muscle Cell Migration