BCL3 anticorps (C-Term)

N° du produit ABIN6655291

Détail du produit anti-BCL3 anticorps

Antigène: BCL3 (B-Cell CLL/lymphoma 3 (BCL3))

Épitope: C-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BCL3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Purification: This affinity purified antibody is directed against human BCL3. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human.

Immunogène:

Immunogen: Anti-BCL3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Term portion of human BCL3 conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:200

Application Note: Anti-BCL-3 Antibody has been tested in Western Blot, ELISA, and Immunofluorescence. Expect a band at 47.5 kDa in western blot using appropriate lysates. Positive control used recombinant BCL3 protein, HEK293T overexpressing BCL3 or U87 whole cell lysates at 1 μg/mL in WB, and U87MG with PFA at 10 μg/mL in IF. Although not tested, this antibody is likely functional in immunohistochemistry, flow cytometry, and immunoprecipitation.

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 5.0 μg/mL

Flow Cytometry Dilution: User Optimized

Western Blot Dilution: 1.0 μg/mL

IF Microscopy Dilution: 10 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Détails sur BCL3

Antigène: BCL3 (B-Cell CLL/lymphoma 3 (BCL3))

Autre désignation: BCL3 (BCL3 Produits)

Synonymes: anticorps BCL3, anticorps AI528691, anticorps Bcl-3, anticorps BCL4, anticorps D19S37, anticorps B-cell CLL/lymphoma 3, anticorps B cell leukemia/lymphoma 3, anticorps BCL3, anticorps Bcl3

Sujet:

Synonyms: rabbit anti-BCL3 antibody, rabbit anti-BCL-3 Antibody, BCL 3, B-cell lymphoma 3 protein, B-cell lymphoma 3 protein Antibody

Background: BCL3 belongs to the ANKRD family. The protein contains seven ankyrin repeats, which are closely related to those found in I kappa B proteins. BCL3 functions as a transcriptional co-activator, regulator of transcriptional activation, promotor of transcription, and inhibitor of nuclear translocation of NF-kappa B. It is a proto-oncogene identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. BCL3 contributes to the regulation of cell proliferation. Therefore, BCL3 may be associated with B-cell chronic lymphocytic leukemia, lymphoma, or other hematologic diseases. Anti-BCL3 Antibody is useful for researchers interested in Cancer Research, NF-Kappa B Pathway, and TNF Signaling Pathway.

Gene Name: BCL3

ID gène: 602

NCBI Accession: NP_005169

UniProt: P20749

Pathways: Signalisation NF-kappaB, Maintenance of Protein Location, Protein targeting to Nucleus