CD8 alpha anticorps (PE)

N° du produit ABIN6655404

N° du produit (Fournisseur): 200-308-n61

Cet anticorps Souris Monoclonal détecte spécifiquement CD8 alpha dans FACS et IHC. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour CD8 alpha anticorps (PE) (ABIN6655404)

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8 alpha est conjugé à/à la PE

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: HIT8a

Détail du produit anti-CD8 alpha anticorps (PE)

N° du produit (Fournisseur): 200-308-n61

Fournisseur: Rockland

Fonction: CD8a Phycoerythrin Antibody

Réactivité croisée (Details): Reactivity is observed against human CD8a, Marmoset, and chimpanzee.

Purification: Phycoerythrin conjugated CD8a Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD8a.

Stérilité: Sterile filtered

Immunogène: Anti-CD8a Antibody (Monoclonal) was produced by repeated immunizations with CD8a antigen.

Isotype: IgG1 kappa

Labeling Ratio: 1-2

Information d'application

Indications d'application:

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

Commentaires:

Anti-CD8a is tested in Flow Cytometry and is useful for Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

Preservative: 0.09 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Date de péremption: 6 months

Détails sur CD8 alpha

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Autre désignation: CD8a

Sujet:

Synonyms: T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a

Background: CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Gene Name: CD8A

ID gène: 925

NCBI Accession: NP_001139345

UniProt: P01732

Pathways: TCR Signaling