PLK1 anticorps

N° du produit ABIN6655435

N° du produit (Fournisseur): 200-901-mh7s

L’anticorps Poulet Polyclonal anti-PLK1 a été validé pour WB, ELISA, IF et FM. Il convient pour détecter PLK1 dans des échantillons de Humain.

Aperçu rapide pour PLK1 anticorps (ABIN6655435)

Antigène: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivité: Humain

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp PLK1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Détail du produit anti-PLK1 anticorps

N° du produit (Fournisseur): 200-901-mh7s

Fournisseur: Rockland

Fonction: PLK1 Antibody

Réactivité croisée (Details): Reactivity occurs against human PLK1.

Purification: This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène: Anti-PLK1 antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to an internal portion of human PLK1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 1:10,000 - 1:50,000

IF_Microscopy_Dilution: 5 μg/mL

Western_Blot_Dilution: 1:500

Commentaires:

Anti-PLK1 antibody has been tested in Western Blot and Immunofluorescence. Expect a band ~68.3kDa in appropriate cell lysates or tissues. Positive controls used are HEK293T or K-562 whole cell lysate in WB, and HeLa cells in IF. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.002 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.0

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur PLK1

Antigène: PLK1 (Polo-Like Kinase 1 (PLK1))

Autre désignation: PLK1

Sujet:

Synonyms: Chicken Anti-Polo Like Kinase 1 Antibody, Chicken Anti-PLK1 Antibody, Chicken PLK-1 Antibody, Serine/Threonine-Protein Kinase 13, Polo-Like Kinase 1, STPK13, PLK-1, PLK, Serine/Threonine-Protein Kinase PLK1, Cell Cycle Regulated Protein Kinase, Polo-Like Kinase (Drosophila), Polo (Drosophila)-Like Kinase, EC 2.7.11.21

Background: Polo-like kinases (PLKs) are a family of four serine/threonine protein kinases that belongs to the CDC5/Polo subfamily and are critical regulators of cell cycle progression, mitosis, cytokinesis, elevated levels are found in many different types of cancer, and the DNA damage response. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis, hence, it is a target for cancer therapy. PLK-1, -2 and -3 are ubiquitously expressed, whereas PLK-4 is restricted to a few tissues. Diseases associated with PLK1 include Breast Cancer, Transient Tic Disorder, and Monoclonal Paraproteinemia. Anti-PLK1 Antibody is useful for researchers interested in Cancer Researcher, Cell Cycle Research, and Kinase Activity Research.

Gene Name: PLK1

ID gène: 5347

NCBI Accession: NP_005021

UniProt: P53350

Pathways: Cycle Cellulaire, M Phase