TLR5 anticorps

N° du produit ABIN6655865

Détail du produit anti-TLR5 anticorps

Antigène: TLR5 (Toll-Like Receptor 5 (TLR5))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TLR5 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Réactivité croisée: Humain, Souris

Purification: Anti-TLR5 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-TLR5 from dog, human and mouse based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-TLR5 from other sources has not been determined.

Immunogène:

Immunogen: TLR5 Antibody was produced in mice prepared by repeated immunizations with a synthetic KLH-conjugated peptide corresponding to human TLR5.

Immunogen Type: Peptide

Clone: 85B152-5

Isotype: IgG2a

Information d'application

Indications d'application:

Application Note: Anti-TLR5 Antibody is suitable for use in Flow (I), IHC (p) and WB. Expect a band approximately 97 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Flow Cytometry Dilution: 1-3 μg/10^6 cells

Western Blot Dilution: 1-3 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

0.05 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Détails sur TLR5

Antigène: TLR5 (Toll-Like Receptor 5 (TLR5))

Autre désignation: TLR5 (TLR5 Produits)

Synonymes: anticorps SLEB1, anticorps TIL3, anticorps TLR-5, anticorps sleb1, anticorps til3, anticorps tlr-5, anticorps tlr5, anticorps toll like receptor 5, anticorps toll-like receptor 5, anticorps toll like receptor 5 L homeolog, anticorps toll-like receptor 5b, anticorps TLR5, anticorps Tlr5, anticorps tlr5.L, anticorps tlr-5, anticorps tlr5b

Sujet:

Synonyms: TIL3, Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3

Background: Anti-TLR5 Antibody detects human TLR5. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Thirteen homologs of TLRs (TLR1-13) have been described. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens. Anti-TLR5 Antibody is ideal for investigators involved in cytokines and growth factor research.

Gene Name: TLR5

ID gène: 7100

NCBI Accession: NP_003259

UniProt: O60602

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades