TJP1 anticorps

N° du produit ABIN6655950

N° du produit (Fournisseur): 600-401-gu7s

Cet anticorps Lapin Polyclonal détecte spécifiquement TJP1 dans WB, IHC, ELISA, IF, FACS, FM et MA. Il présente une réactivité envers Humain et a été mentionné dans 1 publication.

Aperçu rapide pour TJP1 anticorps (ABIN6655950)

Antigène: TJP1 (Tight Junction Protein 1 (TJP1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TJP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Détail du produit anti-TJP1 anticorps

N° du produit (Fournisseur): 600-401-gu7s

Fournisseur: Rockland

Fonction: ZO-1 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human ZO-1.

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène: Anti-ZO-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human ZO-1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 10,000-1:50,000

Immunohistochemistry_Dilution: 1:100 - 1:200

Flow_Cytometry_Dilution: User Optimized

IF_Microscopy_Dilution: 10 μg/mL

Western_Blot_Dilution: 1:1000

Commentaires:

Anti-ZO-1 Antibody has been tested in Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, and Flow Cytometry. Expect a band at ~245 and/or 195.5 kDa in western blot using appropriate lysates. Positive control whole cell lysates used A549 and PC3 @ 1μg/mL for WB, CACO2 and PC3 with PFA and MeOH @ 10μg/mL for IF. Positive control cells for FC were PC3 and positive control tissues for IHC was mouse adipose tissue.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Références pour anticorps anti-TJP1 (ABIN6655950)

Singh, Zabbarova, Ikeda, Maranchie, Chermansky, Foley, Hitchens, Yoshimura, Kanai, Kaufman, Tyagi: "Virtual measurements of paracellular permeability and chronic inflammation via color coded pixel-wise T1 mapping." dans: American journal of physiology. Renal physiology, Vol. 319, Issue 3, pp. F506-F514, (2020) (PubMed).

Détails sur TJP1

Antigène: TJP1 (Tight Junction Protein 1 (TJP1))

Autre désignation: ZO-1

Sujet:

Synonyms: rabbit anti-ZO-1 antibody, ZO 1, ZO1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, TJP1

Background: ZO-1, also called TJP1, belongs to the MAGUK family. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. ZO-1 plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. ZO1 may be associated the following disorders, celiac desease, congenital nephrotic syndrome finnish type, and macular degeneration. Anti-ZO-1 Antibody is useful for researchers interested in Apoptosis Research and Insulin Research.

Gene Name: TJP1

ID gène: 7082

NCBI Accession: NP_003248

UniProt: Q07157

Pathways: Carbohydrate Homeostasis, Cell-Cell Junction Organization