ENO2/NSE anticorps

N° du produit ABIN6656045

N° du produit (Fournisseur): 100-401-e00

Cet anticorps anti-ENO2/NSE est un anticorps Lapin Polyclonal détectant ENO2/NSE dans WB, IHC, IF, MA et FM. Adapté pour Humain.

Aperçu rapide pour ENO2/NSE anticorps (ABIN6656045)

Antigène: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ENO2/NSE est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Multiplex Assay (MA), Fluorescence Microscopy (FM)

Détail du produit anti-ENO2/NSE anticorps

N° du produit (Fournisseur): 100-401-e00

Fournisseur: Rockland

Fonction: Neuron specific enolase (NSE) Antibody

Réactivité croisée (Details): This antibody is a monospecific neat serum.

Purification: Anti-NSE antibody directed against human neuron specific enolase protein.

Immunogène: Anti-NSE Antibody was produced by repeated immunizations with recombinant human NSE expressed in E. coli.

Isotype: IgG

Information d'application

Indications d'application:

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:2000

Other: User Optimized

Commentaires:

Anti-Neuron specific enolase (Rabbit) antibody has been tested by Western Blot, IHC, ICC, and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47kDa in size corresponding to NSE protein by western blotting.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur ENO2/NSE

Antigène: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Autre désignation: Neuron specific enolase (NSE)

Sujet:

Synonyms: Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, NSE

Background: NSE antibody detects NSE protein. Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers. NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury. Anti-neuron specific enolase (NSE) antibody is ideal for investigators involved in Cell Signaling, Neuroscience, and Signal Transduction research.

Gene Name: ENO2

ID gène: 2026

UniProt: P09104