PDIA3 anticorps

N° du produit ABIN6656047

N° du produit (Fournisseur): 200-301-f26

L’anticorps Souris Monoclonal anti-PDIA3 a été validé pour WB, IHC, IF et IP. Il convient pour détecter PDIA3 dans des échantillons de Humain.

Aperçu rapide pour PDIA3 anticorps (ABIN6656047)

Antigène: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PDIA3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: Map-ERP57

Détail du produit anti-PDIA3 anticorps

N° du produit (Fournisseur): 200-301-f26

Fournisseur: Rockland

Fonction: Erp57 (Grp58) Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with Erp57 (Grp58) from Human, Mouse, Bovine, Canine, Guinea pig, Hamster, Monkey, Pig, Rabbit, and Rat based on 100 % homology with the immunizing sequence.

Purification: Anti-Erp57 (Grp58) Antibody was purified by Protein G chromatography.

Stérilité: Sterile filtered

Immunogène: Erp57 (Grp58) Antibody was produced in mice by repeated immunizations with a human Recombinant Erp57 (Grp58).

Isotype: IgG1

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1:2000

Commentaires:

Anti-EC SOD Antibody has been tested in WB, IP, IF, and IHC. Expect a band approximately ~57kDa in specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur PDIA3

Antigène: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Autre désignation: Erp57 (Grp58)

Sujet:

Synonyms: ERp60, ERp61, Grp57, Grp58, P58, PDIA3, PI PLC, Protein disulfide-isomerase A3, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, Endoplasmic reticulum resident protein 60

Background: ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs. It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glucosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface. ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus.

Gene Name: PDIA3

ID gène: 2923

NCBI Accession: NP_005304

UniProt: P30101

Pathways: Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis