GRP78 anticorps

N° du produit ABIN6656070

N° du produit (Fournisseur): 200-301-f36

Cet anticorps Souris Monoclonal détecte spécifiquement GRP78 dans WB, IF et FM. Il présente une réactivité envers Humain et a été mentionné dans 1 publication.

Aperçu rapide pour GRP78 anticorps (ABIN6656070)

Antigène: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GRP78 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Clone: 1H11-1H7

Détail du produit anti-GRP78 anticorps

N° du produit (Fournisseur): 200-301-f36

Fournisseur: Rockland

Fonction: GRP78 Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with GRP78 from Human, Mouse, Rat, Cow, Cryphonectria parasitica, Hamster, Monkey, Rabbit, and Xenopus based on 100 % homology with the immunizing sequence.

Purification: Anti-GRP78 Antibody was purified by Protein G chromatography.

Stérilité: Sterile filtered

Immunogène: Grp78 Antibody was produced in mice by repeated immunizations with a his-tagged human GRP78.

Isotype: IgG2b

Information d'application

Indications d'application:

IF_Microscopy_Dilution: 1:100

Western_Blot_Dilution: 1:1000

Commentaires:

Anti-GRP78 Antibody has been tested by western blot and immunocytochemistry/immunofluorescence. Expect a band approximately ~78kDa corresponding to specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-GRP78 (ABIN6656070)

Hong, Tseng, Wala, Kim, Kynnap, Doshi, Kugener, Sandoval, Howard, Li, Yang, Tillgren, Ghandi, Sayeed, Deasy, Ward, McSteen, Labella, Keskula, Tracy, Connor, Clinton, Church, Crompton, Janeway et al.: "Renal medullary carcinomas depend upon SMARCB1 loss and are sensitive to proteasome inhibition. ..." dans: eLife, Vol. 8, (2019) (PubMed).

Détails sur GRP78

Antigène: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Autre désignation: GRP78

Sujet:

Synonyms: BiP, Grp78, HspA5, MIF2, immunoglobulin heavy chain binding protein, 78 kDa glucose-regulated protein, Heat shock 70 kDa protein 5

Background: GRP78 is a ubiquitously expressed, 78- kDa glucose- regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed. GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis. Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival. In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress, and its protein levels are reduced in the brains of Alzheimer's patients. Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs.

Gene Name: Hspa5

ID gène: 3309

NCBI Accession: NP_005338

UniProt: P11021

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling