Choline Acetyltransferase anticorps

N° du produit ABIN6656077

Détail du produit anti-Choline Acetyltransferase anticorps

Antigène: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Choline Acetyltransferase est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Réactivité croisée: Oiseau, Poulet, Cobaye, Humain, Souris, Non-Human Primate

Réactivité croisée (Details): Cross reactivity with ChAT from other species has not been determined

Purification: Anti-ChAT antibody is directed against human ChAT protein. The antibody was affinity purified from monospecific antiserum by immunoaffinity purification. . However, reactivity is also expected from the following species based on 100% sequence homology: chicken, guinea pig, mouse, and non-human primates.

Immunogène:

Immunogen: Anti-ChAT Antibody was produced in rabbit by repeated immunizations with native choline acetyltransferase purified from human placenta.

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:100

Application Note: Anti-ChAT antibody is suitable for use in ELISA, Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a ~ 68/70 kDa band corresponding to choline acetyltransferase protein by Western Blot in the appropriate lysate or tissue.

Western Blot Dilution: 1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 5 mg BSA

0.02 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Références pour anticorps anti-Choline Acetyltransferase (ABIN6656077)

Liu, Kim, Kim, Zhang, Bao, Denaxa, Lim, Kim, Liu, Wickersham, Pachnis, Hattar, Song, Brown, Blackshaw: "Lhx6-positive GABA-releasing neurons of the zona incerta promote sleep." dans: Nature, Vol. 548, Issue 7669, pp. 582-587, (2017) (PubMed).

Détails sur Choline Acetyltransferase

Antigène: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Autre désignation: ChAT (Choline Acetyltransferase) (CHAT Produits)

Synonymes: anticorps CHOACTASE, anticorps CMS1A, anticorps CMS1A2, anticorps ChoAcTase, anticorps B230380D24Rik, anticorps choline O-acetyltransferase, anticorps choline O-acetyltransferase a, anticorps choline acetyltransferase, anticorps carnitine O-acetyltransferase, anticorps CHAT, anticorps chata, anticorps Chat, anticorps PGTG_00551, anticorps LOC100533322

Sujet:

Synonyms: acetyl CoA:choline O-acetyltransferase, CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O-acetyltransferase, CLAT, CMS1A, CMS1A2

Background: ChAT antibody recognizes choline acetyltransferase which is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. ChAT is found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome. Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects. It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function. Anti-ChAT antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: CHAT

ID gène: 1103

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development