DNAJB1 anticorps

N° du produit ABIN6656695

N° du produit (Fournisseur): 200-301-f56

L’anticorps Souris Monoclonal anti-DNAJB1 a été validé pour WB, ELISA, IHC, IP, IF et FM. Il convient pour détecter DNAJB1 dans des échantillons de Humain.

Aperçu rapide pour DNAJB1 anticorps (ABIN6656695)

Antigène: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DNAJB1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Clone: 3B9-E6

Détail du produit anti-DNAJB1 anticorps

N° du produit (Fournisseur): 200-301-f56

Fournisseur: Rockland

Fonction: HSP40 (HDJ1) Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with Hsp40 from Human, Mouse, and Rat based on 100 % homology with the immunizing sequence.

Purification: Anti-Hsp40 Antibody was purified by Protein G chromatography.

Stérilité: Sterile filtered

Immunogène: Hsp40 Antibody was produced in mice by repeated immunizations raised against recombinant Protein Hsp40 (Hdj1).

Isotype: IgG1

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:200

Immunohistochemistry_Dilution: User Optimized

IF_Microscopy_Dilution: 10 μg/mL

Western_Blot_Dilution: 1:2000

Commentaires:

Anti-Hsp40 Antibody is tested for use in WB, IP, IHC, IF, and ELISA. Expect a band approximately ~40kDa protein corresponding to the molecular mass of Hsp40 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur DNAJB1

Antigène: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Autre désignation: Hsp40 (Hdj1)

Sujet:

Synonyms: DNAJ1, NDAJB1, HDJ1, Hsp40, HSPF1, DnaJ homolog subfamily B member 1, Dna J protein homolog 1, Heat shock 40 kDa protein 1, HSP40, heat shock protein 40, Human DnaJ protein 1, hDj-1

Background: Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of Hsp70 function. HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins. The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1. Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding.

Gene Name: DNAJB1

ID gène: 3337

NCBI Accession: NP_006136

UniProt: P25685