TLR5 anticorps

N° du produit ABIN6656728

N° du produit (Fournisseur): 200-401-i28

L’anticorps Lapin Polyclonal anti-TLR5 a été validé pour WB, IHC et FACS. Il convient pour détecter TLR5 dans des échantillons de Humain.

Aperçu rapide pour TLR5 anticorps (ABIN6656728)

Antigène: TLR5 (Toll-Like Receptor 5 (TLR5))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TLR5 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-TLR5 anticorps

N° du produit (Fournisseur): 200-401-i28

Fournisseur: Rockland

Fonction: TLR5 Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-TLR5 from human, rat and mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-TLR5 Antibody was purified by Protein G chromatography.

Immunogène: TLR5 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a KLH-conjugated peptide corresponding to internal amino acids on human TLR5 protein.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry_Dilution: 10 μg/mL (paraffin)

Flow_Cytometry_Dilution: 1-2 μg/10^6 cells

Western_Blot_Dilution: 1-3 μg/mL

Commentaires:

Anti-TLR5 Antibody is tested for use in WB, Flow, Flow-CS, IHC, and IHC-P. Expect a band approximately 97kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.05 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur TLR5

Antigène: TLR5 (Toll-Like Receptor 5 (TLR5))

Autre désignation: TLR5

Sujet:

Synonyms: TIL3, Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3

Background: Anti-TLR5 Antibody detects human TLR5. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Thirteen homologs of TLRs (TLR1-13) have been described. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens. Anti-TLR5 Antibody is ideal for investigator involved in cytokine and growth factor research.

Gene Name: TLR5

ID gène: 7100

NCBI Accession: NP_003259

UniProt: O60602

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades