NOS1 anticorps (AA 1-181)

N° du produit ABIN6657480

N° du produit (Fournisseur): 200-301-p43

Cet anticorps Souris Monoclonal détecte spécifiquement NOS1 dans WB et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour NOS1 anticorps (AA 1-181) (ABIN6657480)

Antigène: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NOS1 est non-conjugé

Application: Western Blotting (WB), ELISA

Clone: NOS-B1

Détail du produit anti-NOS1 anticorps

Épitope: AA 1-181

N° du produit (Fournisseur): 200-301-p43

Fournisseur: Rockland

Fonction: Nitric Oxide Synthase (NOS1) Antibody

Réactivité croisée (Details): This product reacts with human, rat, goat, and pig.

Purification: Anti-NOS1 antibody was purified from mouse ascites by protein A chromatography.

Immunogène: Nitric Oxide Synthase (NOS1) antibody was produced in mice by repeated immunizations with recombinant protein corresponding to neuronal NOS fragment (amino acids 1-181) from rat brain.

Isotype: IgG1

Information d'application

Indications d'application:

ELISA_Dilution: 1:5000

Western_Blot_Dilution: 0.5 μg/mL

Commentaires:

Anti-NOS1 is tested for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~161 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 1.0 mL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur NOS1

Antigène: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Autre désignation: Nitric Oxide Synthase (NOS1)

Sujet:

Synonyms: NOS, bNOS, nNOS, IHPS1, N-NOS, NC-NOS, Neuronal NOS, Peptidyl-cysteine S-nitrosylase NOS1

Background: Nitric Oxide Synthase 1(NOS1,neuronal NOS,nNOS1) is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. It may be involved in neuronal cell death and damage in neurological illness. nNOS1 localized to the 12q24.2 region of human chromosome 12. It splice variant, expressed in testis, that encodes an NH2-terminal truncated protein of 1098 amino acids. nNOS cDNA clones were shown to contain different 5' terminal exons spliced to a common exon 2. Genomic cloning and sequence analysis demonstrate that the unique exons are positioned within 300 bp of each other but separated from exon 2 by an intron that is at least 20 kb in length. The neuronal isoform of nitric oxide synthase is highly expressed in mammalian skeletal muscle, it suggested a specific role for NOS1 in the local metabolic inhibition of alpha-adrenergic vasoconstriction in active skeletal muscle. The novel gaseous neuromediator nitric oxide is thought to play an important role in development and plasticity. Despite this, gene-knockout mice lacking neuronal (Type I) nitric oxide synthase exhibit relatively normal brain development and behavior. This antibody is suitable for researchers interested in apoptosis research, cell death, and TNF pathways.

Gene Name: NOS1

ID gène: 4842

NCBI Accession: NP_000611

UniProt: P29475

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction