Cytokeratin 18 anticorps

N° du produit ABIN6657540

N° du produit (Fournisseur): 200-301-p33

Cet anticorps Souris Monoclonal détecte spécifiquement Cytokeratin 18 dans WB, IHC et IF. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Cytokeratin 18 anticorps (ABIN6657540)

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 18 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: CY-90

Détail du produit anti-Cytokeratin 18 anticorps

N° du produit (Fournisseur): 200-301-p33

Fournisseur: Rockland

Fonction: Cytokeratin Peptide 18 Antibody

Réactivité croisée (Details): This product reacts with human and rat.

Purification: Anti-Cytokeratin Peptide 18 antibody was purified from mouse ascites by protein A chromatography.

Immunogène: Cytokeratin Peptide 18 antibody was produced in mice by repeated immunizations with the human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.

Isotype: IgG1

Information d'application

Indications d'application:

Immunohistochemistry_Dilution: 0.5-2 μg/mL

Western_Blot_Dilution: 0.5-1 μg/mL

Commentaires:

Anti-Cytokeratin Peptide 18 is tested for Immunohistochemistry-F, Immunohistochemistry-P, Immunofluorescence, and Western Blot. Expect a band approximately ~48 kDa corresponding to the appropriate cell lysate or extract. western blot tested in human. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 1.0 mL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Cytokeratin 18

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Autre désignation: Cytokeratin Peptide 18

Sujet:

Synonyms: K18, Keratin-18, CK-18, Cytokeratin-18, CYK18, Cell proliferation-inducing gene 46 protein

Background: Intermediate filaments (IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18(KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis. This antibody is suitable for researchers interested in cancer research, cardiovascular diseases, and cytoskeletal signaling research.

Gene Name: KRT18

ID gène: 3875

NCBI Accession: NP_000215

UniProt: P05783

Pathways: Apoptose, Caspase Cascade in Apoptosis