PIK3CD anticorps (C-Term)

N° du produit ABIN6657601

N° du produit (Fournisseur): 100-401-862

L’anticorps Lapin Polyclonal anti-PIK3CD a été validé pour WB, ELISA et IP. Il convient pour détecter PIK3CD dans des échantillons de Souris. Il y a 1 publication disponible.

Aperçu rapide pour PIK3CD anticorps (C-Term) (ABIN6657601)

Antigène: PIK3CD (Phosphoinositide-3-Kinase, Catalytic, delta Polypeptide (PIK3CD))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PIK3CD est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-PIK3CD anticorps

Épitope: C-Term

N° du produit (Fournisseur): 100-401-862

Fournisseur: Rockland

Fonction: PI3-kinase p110 delta Antibody

Réactivité croisée (Details): Reactivity occurs against mouse PI3K p110d subunit.

Purification: This antibody was prepared from whole rabbit antiserum by delipidation and defibrination.

Stérilité: Sterile filtered

Immunogène: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of mouse PI3K p110d. This sequence is identical in both mouse and human.

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 1:4,000 - 1:20,000

Western_Blot_Dilution: 1:5,000

Other: User Optimized

Commentaires:

Anti-PI3 kinase p110 delta subunit antibody has been tested for use in ELISA, western blotting and immunoprecipitation.  Reactivity in other immunoassays is unknown.  A mouse whole cell splenic lysate is suitable for use as a positive control.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-PIK3CD (ABIN6657601)

Jou, Carpino, Takahashi, Piekorz, Chao, Carpino, Wang, Ihle: "Essential, nonredundant role for the phosphoinositide 3-kinase p110delta in signaling by the B-cell receptor complex." dans: Molecular and cellular biology, Vol. 22, Issue 24, pp. 8580-91, (2003) (PubMed).

Détails sur PIK3CD

Antigène: PIK3CD (Phosphoinositide-3-Kinase, Catalytic, delta Polypeptide (PIK3CD))

Autre désignation: PI3-kinase p110 delta

Sujet:

Synonyms: rabbit anti-PI3-kinase p110 delta Antibody, p110D antibody, p110delta antibody, Phosphatidylinositol 3 kinase catalytic delta polypeptide antibody, Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform antibody, PI3K antibody, PIK3CD antibody

Background: Phosphoinositide 3-kinases (PI3Ks) generate 3-phosphoinositide lipids in cell membranes.  A variety of intracellular target proteins interact with these lipids via specific lipid-binding modules and, as a consequence, undergo changes in their localization and/or activity. In this way, PI3Ks participate in the regulation of mitogenesis, differentiation, survival, intracellular vesicular transport, cytoskeletal reorganization, and motility.  Tyrosine kinases and Ras use PI3Ks as essential intracellular signal relay molecules. PI3Ks are heterodimeric enzymes consisting of a regulatory subunit in complex with a p110 catalytic subunit. Mammmals have genes encoding three distinct catalytic subunits (p110α, p110ß, and p110δ and three regulatory subunits (p85α, p85ß, and p55δ). All of the p110 isoforms are capable of interacting with each type of regulatory subunit. They are also similarly recruited to phosphotyrosine complexes and have, at least in vitro, the same lipid substrate specificity. However, it is becoming increasingly clear that PI3K isoforms differ in their interaction with Ras and regulation of lipid kinase activity, and in their protein kinase activities. Several groups have provided evidence that p110 isoforms have nonredundant functions in the regulation of cell proliferation, survival, actin cytoskeleton reorganization, and migration downstream of given receptors. This antibody is specific for the carboxy teminal end of p110d that is expressed predominantly in leukocytes.

Gene Name: PIK3CD

ID gène: 18707

UniProt: O35904

Pathways: BCR Signaling, L'effet Warburg