HSF1 anticorps (AA 425-439) (PerCP)

N° du produit ABIN6657640

N° du produit (Fournisseur): 200-565-w89

L’anticorps Rat Monoclonal anti-HSF1 a été validé pour WB, ELISA, IP et GS. Il convient pour détecter HSF1 dans des échantillons de Souris.

Aperçu rapide pour HSF1 anticorps (AA 425-439) (PerCP) (ABIN6657640)

Antigène: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp HSF1 est conjugé à/à la PerCP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Gel Shift (GS)

Clone: 4B4

Détail du produit anti-HSF1 anticorps (PerCP)

Épitope: AA 425-439

N° du produit (Fournisseur): 200-565-w89

Fournisseur: Rockland

Fonction: HSF1 Antibody PerCP

Purification: Anti-HSF1 Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

Stérilité: Sterile filtered

Immunogène: Anti-HSF1 Antibody was produced by repeated immunization of rats with a purified recombinant mouse HSF1 protein, epitope mapping to amino acids 425-439.

Isotype: IgG1

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:10,000

Gel_Shift_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Commentaires:

Anti-HSF1 PerCP Conjugated Antibody is suitable for Western Blots, Immunoprecipitation, ELISA, Gel Mobility Shift Assay and Immunocytochemistry. Expect a band approximately ~85kDa protein in unstressed cell lysates, and a 95 kDa protein in heat shocked cell lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur HSF1

Antigène: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Autre désignation: HSF1

Sujet:

Synonyms: HSTF1, Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1

Background: HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11). Anti-HSF1 Antibody is ideal for research in Genetics, Transcription, Cell Signaling and pathways including ERK and MAPK.

Gene Name: Hsf1

ID gène: 15499

NCBI Accession: NP_032322

UniProt: P38532