CD8 alpha anticorps (APC)

N° du produit ABIN6657876

N° du produit (Fournisseur): 200-526-n77

Cet anticorps Rat Monoclonal détecte spécifiquement CD8 alpha dans FACS, IHC et IP. Il présente une réactivité envers Souris.

Aperçu rapide pour CD8 alpha anticorps (APC) (ABIN6657876)

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8 alpha est conjugé à/à la APC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: 53-6-7

Détail du produit anti-CD8 alpha anticorps (APC)

N° du produit (Fournisseur): 200-526-n77

Fournisseur: Rockland

Fonction: CD8a Allophycocyanin Antibody

Réactivité croisée (Details): Cross reactivity with CD8a from other sources has not been tested.

Purification: Allophycocyanin conjugated CD8a Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD8a.

Stérilité: Sterile filtered

Immunogène: Anti-CD8a Antibody (Monoclonal) was produced by repeated immunizations with CD8a antigen.

Isotype: IgG2a kappa

Labeling Ratio: 1-2

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

Commentaires:

Anti-CD8a is tested for FLOW and useful for Immunohistochemistry and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Date de péremption: 6 months

Détails sur CD8 alpha

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Autre désignation: CD8a

Sujet:

Synonyms: T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, CD8a, Cd8a, Lyt-2, Lyt2

Background: CD8a antibody reacts with the 32-34 kDa alpha subunit of mouse CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor in antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.The 53-6.7 antibody is widely used as a phenotypic marker for mouse CD8a expression on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells.

Gene Name: Cd8a

ID gène: 12525

NCBI Accession: NP_001074579

UniProt: P01731

Pathways: TCR Signaling