CD8 alpha anticorps (PE)

N° du produit ABIN6657880

N° du produit (Fournisseur): 200-508-n77

L’anticorps anti-CD8 alpha Monoclonal Rat est utilisé pour la détection de CD8 alpha dans des échantillons de Souris. Il a été validé pour FACS, IHC et IP.

Aperçu rapide pour CD8 alpha anticorps (PE) (ABIN6657880)

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8 alpha est conjugé à/à la PE

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: 53-6-7

Détail du produit anti-CD8 alpha anticorps (PE)

N° du produit (Fournisseur): 200-508-n77

Fournisseur: Rockland

Fonction: CD8a Phycoerythrin Antibody

Réactivité croisée (Details): Cross reactivity with CD8a from other sources has not been tested.

Purification: Phycoerythrin conjugated CD8a Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD8a.

Stérilité: Sterile filtered

Immunogène: Anti-CD8a Antibody (Monoclonal) was produced by repeated immunizations with Mouse thymus or spleen .

Isotype: IgG2a kappa

Labeling Ratio: 1-2

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

Commentaires:

Anti-CD8a is tested in FLOW and useful for Immunohistochemistry and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.09 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Date de péremption: 6 months

Détails sur CD8 alpha

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Autre désignation: CD8a

Sujet:

Synonyms: T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, CD8a, Cd8a, Lyt-2, Lyt2

Background: CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Gene Name: Cd8a

ID gène: 12525

NCBI Accession: NP_001074579

UniProt: P01731

Pathways: TCR Signaling