SIX3 anticorps (Internal Region)

N° du produit ABIN6657911

Détail du produit anti-SIX3 anticorps

Antigène: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Épitope: Internal Region

Reactivité: Souris

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Cet anticorp SIX3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Purification: This product was purified by Protein A chromatography from monospecific antiserum.  This antibody reacts with mouse Six3.   Expect partial reactivity with Six3 from chicken and Xenopus sources based on 95% and 85% homologies, respectively.  Cross-reactivity with Six3 from other sources has not been determined.

Immunogène:

Immunogen: This Protein A purified antibody was prepared from whole guinea pig serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Six3 protein.

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:200

Application Note: This Protein A purified antibody has been tested for use in ELISA, immunofluorescence microscopy and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 37 kDa in size corresponding to Six3 by western blotting in the appropriate cell lysate or extract.

ELISA Dilution: 1:5,000 - 1:25,000

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 1:200 - 1:1,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Références pour anticorps anti-SIX3 (ABIN6657911)

Lipiec, Bem, Koziński, Chakraborty, Urban-Ciećko, Zajkowski, Dąbrowski, Szewczyk, Toval, Ferran, Nagalski, Wiśniewska: "TCF7L2 regulates postmitotic differentiation programmes and excitability patterns in the thalamus." dans: Development (Cambridge, England), Vol. 147, Issue 16, (2021) (PubMed).

OBrien, Guo, Bahrami-Samani, Park, Hasso, Lee, Fang, Kim, Guo, Hong, Peterson, Lozanoff, Raviram, Ren, Fogelgren, Smith, Valouev, McMahon: "Transcriptional regulatory control of mammalian nephron progenitors revealed by multi-factor cistromic analysis and genetic studies." dans: PLoS genetics, Vol. 14, Issue 1, pp. e1007181, (2018) (PubMed).

Détails sur SIX3

Antigène: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Autre désignation: SIX3 (SIX3 Produits)

Synonymes: anticorps HPE2, anticorps SIX3, anticorps cb347, anticorps six3.2, anticorps six6, anticorps E130112M24Rik, anticorps Six3a, anticorps Six3alpha, anticorps Six3b, anticorps Six3beta, anticorps six3, anticorps six3.1, anticorps wu:fc10a08, anticorps SIX homeobox 3, anticorps SIX homeobox 3b, anticorps sine oculis-related homeobox 3, anticorps SIX homeobox 3a, anticorps sine oculis homeobox homolog 3, anticorps SIX3, anticorps six3b, anticorps Six3, anticorps six3a, anticorps six3

Sujet:

Synonyms: guinea pig anti-SIX 3 antibody, Six3, Sine oculis homeobox homolog 3

Background: Six3 (also known as sine oculis homeobox homolog 3) is involved in the development of the visual system and forebrain.  Six3 is a nuclear protein that is reported to exist in two forms by alternative splicing of the gene product.  Six3 is first expressed at E6.5 of mouse embryonic development around the anterior border. At E8.5, expression is found over the anterior neural plate. At E9.5, it is in the diencephalic part of the ventral forebrain, optic vesicles, olfactory placodes and Rathke's pouch. In later stages, Six3 is present in hypothalamus, eyes and pituitary.

Gene Name: SIX3

ID gène: 20473, 59939908

UniProt: Q62233

Pathways: Protein targeting to Nucleus