CD68 anticorps (Internal Region)

N° du produit ABIN6658039

N° du produit (Fournisseur): 600-401-r10

L’anticorps Lapin Polyclonal anti-CD68 a été validé pour FACS, IHC, WB, IF et FM. Il convient pour détecter CD68 dans des échantillons de Souris. Il y a 2+ publications disponibles.

Aperçu rapide pour CD68 anticorps (Internal Region) (ABIN6658039)

Antigène: CD68 (CD68 Molecule (CD68))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CD68 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Détail du produit anti-CD68 anticorps

Épitope: Internal Region

N° du produit (Fournisseur): 600-401-r10

Fournisseur: Rockland

Fonction: Cd68 Antibody

Réactivité croisée (Details): Anti-CD68 antibody is directed against mouse CD68 protein supplied in the buffer above with 4mg trehalose.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogène: CD68 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse CD68.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry_Dilution: 0.5-1.0 μg/mL

Flow_Cytometry_Dilution: 1-3 μg/1 million cells

IF_Microscopy_Dilution: 5 μg/mL

Western_Blot_Dilution: 0.1-0.5 μg/mL

Commentaires:

Suggested Applications: Other
Anti-CD68 is tested for IHC-P, IHC-Fr, IF, Flow Cytometry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~34.8 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 200μL

Buffer: Optional[Buffer]: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-CD68 (ABIN6658039)

Adrian, Weber, Tsai, Glock, Kahn, Phu, Cheung, Meilandt, Rose, Hoogenraad: "Polarized microtubule remodeling transforms the morphology of reactive microglia and drives cytokine release." dans: Nature communications, Vol. 14, Issue 1, pp. 6322, (2023) (PubMed).

Abuelezz, Hendawy, Magdy: "Targeting Oxidative Stress, Cytokines and Serotonin Interactions Via Indoleamine 2, 3 Dioxygenase by Coenzyme Q10: Role in Suppressing Depressive Like Behavior in Rats." dans: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, Vol. 12, Issue 2, pp. 277-291, (2018) (PubMed).

Détails sur CD68

Antigène: CD68 (CD68 Molecule (CD68))

Autre désignation: CD68

Sujet:

Synonyms: Macrosialin, Gp110, CD68

Background: CD68, cluster of differentiation, is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. The CD68 gene is mapped to 17p13.1. Immunohistochemistry can be used to identify the presence of CD68, which is found in the cytoplasmic granules of a range of different blood cells. It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukaemia (the latter being CD68 negative). Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease. This antibody is suitable for researchers interested in cancer research.

Gene Name: CD68

UniProt: P31996