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MBP anticorps

L’anticorps Poulet Polyclonal anti-MBP a été validé pour WB, IF et FM. Il convient pour détecter MBP dans des échantillons de Rat.
Rockland
N° du produit ABIN6658054
N° du produit (Fournisseur): 200-901-d81

Aperçu rapide pour MBP anticorps (ABIN6658054)

Antigène

Voir toutes MBP Anticorps
MBP (Myelin Basic Protein (MBP))

Reactivité

  • 118
  • 36
  • 36
  • 21
  • 13
  • 10
  • 8
  • 6
  • 6
  • 5
  • 5
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  • 1
  • 1
  • 1
Rat

Hôte

  • 82
  • 36
  • 9
  • 6
  • 5
  • 1
Poulet

Clonalité

  • 86
  • 50
  • 1
Polyclonal

Conjugué

  • 92
  • 9
  • 7
  • 7
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp MBP est non-conjugé

Application

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  • 7
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Western Blotting (WB), Immunofluorescence (IF), Fluorescence Microscopy (FM)
  • N° du produit (Fournisseur)

    200-901-d81

    Fournisseur

    Rockland

    Fonction

    Myelin Basic Protein (MBP) Antibody

    Réactivité croisée (Details)

    Anti-MBP antibody is directed against Myelin basic protein.

    Purification

    The antibody is a total IgY fraction.

    Immunogène

    Anti-Myelin basic protein Antibody was produced in chicken by repeated immunizations with three peptide sequences conserved in higher vertebrate MBP proteins.

    Isotype

    IgG
  • Indications d'application

    IF_Microscopy_Dilution: 1:500

    Western_Blot_Dilution: 1:5000

    Commentaires

    Anti-Myelin Basic Protein antibody is tested for use in Western Blotting and IF. Expect a bands of approximately 18 kDa and 22 kDa in size corresponding to MBP proteins in Western blots of rat brain homogenate. Specific conditions for reactivity should be optimized by the end user.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

    Date de péremption

    12 months
  • Antigène

    MBP (Myelin Basic Protein (MBP))

    Sujet

    Synonyms: Myelin A1 protein, Myelin membrane encephalitogenic protein, Myelin basic protein, MBP, Myelin basic protein S, MBP S antibody

    Background: Myelin Basic Protein antibody detects MBP. MBP is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago. The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells. In the central nervous system there are there different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typically show two bands on Western blots, one at about 22 kDa and another at about 18 kDa. Anti-MBP antibody is ideal for investigators involved in Cell Signaling, Neuroscience, and Signal Transduction research.

    Gene Name: MDV1

    ID gène

    24547

    NCBI Accession

    NP_001020465

    UniProt

    P02688
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