MBP anticorps
Aperçu rapide pour MBP anticorps (ABIN6658054)
Antigène
Voir toutes MBP AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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N° du produit (Fournisseur)
- 200-901-d81
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Fournisseur
- Rockland
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Fonction
- Myelin Basic Protein (MBP) Antibody
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Réactivité croisée (Details)
- Anti-MBP antibody is directed against Myelin basic protein.
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Purification
- The antibody is a total IgY fraction.
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Immunogène
- Anti-Myelin basic protein Antibody was produced in chicken by repeated immunizations with three peptide sequences conserved in higher vertebrate MBP proteins.
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Isotype
- IgG
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Indications d'application
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IF_Microscopy_Dilution: 1:500
Western_Blot_Dilution: 1:5000
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Commentaires
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Anti-Myelin Basic Protein antibody is tested for use in Western Blotting and IF. Expect a bands of approximately 18 kDa and 22 kDa in size corresponding to MBP proteins in Western blots of rat brain homogenate. Specific conditions for reactivity should be optimized by the end user.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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Date de péremption
- 12 months
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- MBP (Myelin Basic Protein (MBP))
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Sujet
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Synonyms: Myelin A1 protein, Myelin membrane encephalitogenic protein, Myelin basic protein, MBP, Myelin basic protein S, MBP S antibody
Background: Myelin Basic Protein antibody detects MBP. MBP is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago. The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells. In the central nervous system there are there different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typically show two bands on Western blots, one at about 22 kDa and another at about 18 kDa. Anti-MBP antibody is ideal for investigators involved in Cell Signaling, Neuroscience, and Signal Transduction research.
Gene Name: MDV1
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ID gène
- 24547
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NCBI Accession
- NP_001020465
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UniProt
- P02688
Antigène
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