MBP anticorps

N° du produit ABIN6658054

Détail du produit anti-MBP anticorps

Antigène: MBP (Myelin Basic Protein (MBP))

Reactivité: Rat

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp MBP est non-conjugé

Application: Western Blotting (WB), Fluorescence Microscopy (FM)

Réactivité croisée: Boeuf (Vache), Humain, Souris, Rat (Rattus)

Réactivité croisée (Details): Cross reactivity with MBP from other mammalian tissues is expected.

Purification: Anti-MBP antibody is directed against Myelin basic protein.The antibody is a total IgY fraction. The antibody has been directly tested for reactivity in bovine, human, mouse and rat tissues.

Immunogène:

Immunogen: Anti-Myelin basic protein Antibody was produced in chicken by repeated immunizations with three peptide sequences conserved in higher vertebrate MBP proteins.

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Myelin Basic Protein antibody is suitable for use in Western Blotting and IF. Expect a bands of approximately 18 kDa and 22 kDa in size corresponding to MBP proteins in Western blots of rat brain homogenate. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:5000

IF Microscopy Dilution: 1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.20.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Détails sur MBP

Antigène: MBP (Myelin Basic Protein (MBP))

Abstract: MBP Produits

Synonymes: anticorps C76307, anticorps Hmbpr, anticorps R75289, anticorps golli-mbp, anticorps mld, anticorps shi, anticorps Mbps, anticorps cb274, anticorps fj33b11, anticorps mbp, anticorps wu:fj33b11, anticorps wu:fq15b02, anticorps zgc:136630, anticorps MGC115135, anticorps myelin basic protein, anticorps myelin basic protein a, anticorps myelin basic protein L homeolog, anticorps MBP, anticorps Mbp, anticorps mbpa, anticorps mbp, anticorps mbp.L

Sujet:

Synonyms: Myelin A1 protein, Myelin membrane encephalitogenic protein, Myelin basic protein, MBP, Myelin basic protein S, MBP S antibody

Background: Myelin Basic Protein antibody detects MBP. MBP is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago. The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells. In the central nervous system there are there different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typically show two bands on Western blots, one at about 22 kDa and another at about 18 kDa. Anti-MBP antibody is ideal for investigators involved in Cell Signaling, Neuroscience, and Signal Transduction research.

Gene Name: MDV1

ID gène: 24547

NCBI Accession: NP_001020465

UniProt: P02688