GRP78 anticorps

N° du produit ABIN6658056

N° du produit (Fournisseur): 100-401-f38

Cet anticorps Lapin Polyclonal détecte spécifiquement GRP78 dans WB, IF et FM. Il présente une réactivité envers Rat.

Aperçu rapide pour GRP78 anticorps (ABIN6658056)

Antigène: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GRP78 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Détail du produit anti-GRP78 anticorps

N° du produit (Fournisseur): 100-401-f38

Fournisseur: Rockland

Fonction: GRP78 Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with GRP78 from Human, Mouse, Rat, Cow, Fungus (Cryphonectria parasitica), Hamster, Monkey, Rabbit, Xenopus based on 100 % homology with the immunizing sequence.

Purification: Anti-GRP78 Antibody was prepared from monospecific antiserum by delipidation and defibrination.

Stérilité: Sterile filtered

Immunogène: Grp78 Antibody was produced from whole rabbit serum prepared by repeated immunizations with rat GRP78 (Bip) synthetic peptide conjugated to KLH.

Isotype: IgG

Information d'application

Indications d'application:

IF_Microscopy_Dilution: 1:100

Western_Blot_Dilution: 1:1000

Commentaires:

Anti-GRP78 Antibody is tested in WB and IF and suitable for use in IHC and IP Expect a band approximately ~78kda corresponding to the molecular weight of Grp78 protein. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur GRP78

Antigène: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Autre désignation: GRP78

Sujet:

Synonyms: BiP, Grp78, HspA5, MIF2, immunoglobulin heavy chain binding protein, 78 kDa glucose-regulated protein, Heat shock 70 kDa protein 5, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78

Background: GRP78 is a ubiquitously expressed, 78- kDa glucose regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed. GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis. Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival.

Gene Name: HSPA5

ID gène: 25617

NCBI Accession: NP_037215

UniProt: P06761

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling