SHANK3 anticorps (Internal Region)

N° du produit ABIN6658092

N° du produit (Fournisseur): 200-301-g45

Cet anticorps Souris Monoclonal détecte spécifiquement SHANK3 dans WB, IHC, IP, IF et FM. Il présente une réactivité envers Rat.

Aperçu rapide pour SHANK3 anticorps (Internal Region) (ABIN6658092)

Antigène: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SHANK3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Clone: S69

Détail du produit anti-SHANK3 anticorps

Épitope: Internal Region

N° du produit (Fournisseur): 200-301-g45

Fournisseur: Rockland

Fonction: SHANK3 Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with SHANK3 from human, mouse, and rat based on 100 % homology with the immunizing sequence.

Purification: Anti-SHANK3 Antibody was purified by Protein G chromatography.

Stérilité: Sterile filtered

Immunogène: SHANK3 Antibody was produced in mice by repeated immunizations raised against a synthetic peptide corresponding to an internal region of rat Shank3.

Isotype: IgG2b

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: 0.1-1.0 μg/mL

IF_Microscopy_Dilution: 1.0-10 μg/mL

Western_Blot_Dilution: 1-10 μg/mL

Commentaires:

Anti-SHANK3 Antibody is tested for use in WB, IHC, IF, and IP. Expect a band approximately ~190kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur SHANK3

Antigène: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Autre désignation: SHANK3

Sujet:

Synonyms: Shank3, Prosap2, SH3 and multiple ankyrin repeat domains protein 3, Proline-rich synapse-associated protein 2

Background: Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins. Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are also crucial in receptor tyrosine kinase signaling, specifically, Shank3 can mediate Erk-MAPK and P13K signaling which is crucial for tubule formation. Shank3 is also one of the latest genes to be associated with autism. A mutation of a single copy of Shank3 on chromosome 22q13 can result in language and/or social communication disorders.

Gene Name: Shank3

ID gène: 59312

NCBI Accession: NP_067708

UniProt: Q9JLU4

Pathways: Synaptic Membrane, Tube Formation, Regulation of long-term Neuronal Synaptic Plasticity