CGRP anticorps

N° du produit ABIN6658144

Détail du produit anti-CGRP anticorps

Antigène: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CGRP est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Réactivité croisée: Humain, Rat (Rattus)

Purification: Anti-CGRP Monoclonal Antibody is directed against rat CGRP protein. The antibody was purified from concentrated tissue culture supernate by Protein G chromatography. This antibody is specific for human and rat CGRP protein. A BLAST analysis was used to suggest cross-reactivity with CGRP from most mammalian sources based on very high homology with the immunizing sequence. Calcitonin Gene Related Peptide antibody also recognizes rat beta-CGRP and human alpha-CGRP (10-fold less sensitivity compared to rat alpha-CGRP).

Immunogène:

Immunogen: Anti-CGRP Monoclonal Antibody was produced by repeated immunizations with purified rat alpha-CGRP.

Immunogen Type: Recombinant Protein

Clone: 4901

Isotype: IgG2a

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:50-1:100

Application Note: Anti-Calcitonin Gene Related Peptide antibody is suitable for use in ELISA and ICC. This product is not recommended for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Suggested positive controls are dorsal root ganglia, pancreas and gut.

Immunoprecipitation Dilution: 1:50-1:100

ELISA Dilution: 1:5,000

Western Blot Dilution: not recommended

IF Microscopy Dilution: 1:50-1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Références pour anticorps anti-CGRP (ABIN6658144)

Dhandapani, Arokiaraj, Taberner, Pacifico, Raja, Nocchi, Portulano, Franciosa, Maffei, Hussain, de Castro Reis, Reymond, Perlas, Garcovich, Barth, Johnsson, Lechner, Heppenstall: "Control of mechanical pain hypersensitivity in mice through ligand-targeted photoablation of TrkB-positive sensory neurons." dans: Nature communications, Vol. 9, Issue 1, pp. 1640, (2019) (PubMed).

Détails sur CGRP

Antigène: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Autre désignation: Calcitonin Gene Related Peptide (CGRP) (CALCA Produits)

Synonymes: anticorps CALC1, anticorps CGRP, anticorps CGRP-I, anticorps CGRP1, anticorps CT, anticorps KC, anticorps CALC, anticorps CALCA, anticorps CALC3, anticorps ci-ct, anticorps calca, anticorps CA, anticorps CGRP-1, anticorps Calc, anticorps Calc1, anticorps Cgrp, anticorps Ct, anticorps Ctn, anticorps calcitonin, anticorps CAL6, anticorps Cal1, anticorps RATCAL6, anticorps CGRPI, anticorps calc, anticorps cgrp, anticorps zgc:92886, anticorps calcitonin related polypeptide alpha, anticorps calcitonin related polypeptide beta, anticorps calcitonin-related polypeptide alpha, anticorps calcitonin, anticorps calcitonin/calcitonin-related polypeptide, alpha, anticorps calcitonin-related polypeptide beta, anticorps calcitonin gene-related peptide 2, anticorps CALCA, anticorps CALCB, anticorps ci-ct, anticorps calca, anticorps Calca, anticorps LOC100008771

Sujet:

Synonyms: Alpha-type CGRP, Calcitonin gene-related peptide I

Background: CGRP antibody recognizes Calcitonin Gene Related Peptide or CGRP which is a 37 amino acid peptide and is the most potent endogenous vasodilator currently known. CGRP is primarily produced in nervous tissue, however, its receptors are expressed throughout the body. CGRP is also strongly implicated in the vasodilatory effect of endogenous cannabinoid anandamide in the brain. This effect was found to be antagonized by capsazepine. CGRP is also currently a major target of research in regards to factors effecting the onset of migraine headaches. CGRP antibodies have been used successfully in immunoneutralization. Anti-CGRP antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: CALCA

ID gène: 24241

NCBI Accession: NP_001029128

UniProt: P01256

Pathways: Hormone Activity, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, Feeding Behaviour