CGRP anticorps (Calcitonin-Related Polypeptide alpha)

Details for Product anti-CALCA Antibody No. ABIN6658144
Antigène
  • CALC1
  • CGRP
  • CGRP-I
  • CGRP1
  • CT
  • KC
  • CALC
  • CALCA
  • CALC3
  • ci-ct
  • calca
  • CA
  • CGRP-1
  • Calc
  • Calc1
  • Cgrp
  • Ct
  • Ctn
  • calcitonin
  • CAL6
  • Cal1
  • RATCAL6
  • CGRPI
  • calc
  • cgrp
  • zgc:92886
  • calcitonin related polypeptide alpha
  • calcitonin related polypeptide beta
  • calcitonin-related polypeptide alpha
  • calcitonin
  • calcitonin/calcitonin-related polypeptide, alpha
  • calcitonin gene-related peptide 2
  • CALCA
  • CALCB
  • ci-ct
  • calca
  • Calca
  • LOC100008771
Reactivité
Rat (Rattus)
95
61
31
3
2
1
1
1
1
Hôte
Souris
79
24
10
9
4
1
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp CGRP est non-conjugé
9
5
5
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
57
46
33
25
22
15
14
13
11
8
6
6
3
Options
Immunogène

Immunogen: Anti-CGRP Monoclonal Antibody was produced by repeated immunizations with purified rat alpha-CGRP.

Immunogen Type: Recombinant Protein

Clone 4901
Isotype IgG2a
 Réactivité croisée Humain, Rat (Rattus)
Purification Anti-CGRP Monoclonal Antibody is directed against rat CGRP protein. The antibody was purified from concentrated tissue culture supernate by Protein G chromatography. This antibody is specific for human and rat CGRP protein. A BLAST analysis was used to suggest cross-reactivity with CGRP from most mammalian sources based on very high homology with the immunizing sequence. Calcitonin Gene Related Peptide antibody also recognizes rat beta-CGRP and human alpha-CGRP (10-fold less sensitivity compared to rat alpha-CGRP).
Plasmids, Primers & others Plasmids, Primers & others CGRP products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation Calcitonin Gene Related Peptide (CGRP) (CALCA Antibody Extrait)
Sujet

Synonyms: Alpha-type CGRP, Calcitonin gene-related peptide I

Background: CGRP antibody recognizes Calcitonin Gene Related Peptide or CGRP which is a 37 amino acid peptide and is the most potent endogenous vasodilator currently known. CGRP is primarily produced in nervous tissue, however, its receptors are expressed throughout the body. CGRP is also strongly implicated in the vasodilatory effect of endogenous cannabinoid anandamide in the brain. This effect was found to be antagonized by capsazepine. CGRP is also currently a major target of research in regards to factors effecting the onset of migraine headaches. CGRP antibodies have been used successfully in immunoneutralization. Anti-CGRP antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: CALCA

ID gène 24241
NCBI Accession NP_001029128
UniProt P01256
Pathways Hormone Activity, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, Feeding Behaviour
Indications d'application

Immunohistochemistry Dilution: 1:50-1:100

Application Note: Anti-Calcitonin Gene Related Peptide antibody is suitable for use in ELISA and ICC. This product is not recommended for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Suggested positive controls are dorsal root ganglia, pancreas and gut.

Immunoprecipitation Dilution: 1:50-1:100

ELISA Dilution: 1:5,000

Western Blot Dilution: not recommended

IF Microscopy Dilution: 1:50-1:100

Restrictions For Research Use only
Format Liquid
Buffer

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Stock 4 °C,-20 °C
Stockage commentaire Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Images (Fournisseur)
Immunocytochemistry (ICC) image for anti-Calcitonin-Related Polypeptide alpha (CALCA) antibody (ABIN6658144) Immunocytochemistry of Anti-Calcitonin Gene Related Peptide (CGRP) (Mouse) Antibody -...
Produit citée dans: Dhandapani, Arokiaraj, Taberner, Pacifico, Raja, Nocchi, Portulano, Franciosa, Maffei, Hussain, de Castro Reis, Reymond, Perlas, Garcovich, Barth, Johnsson, Lechner, Heppenstall: "Control of mechanical pain hypersensitivity in mice through ligand-targeted photoablation of TrkB-positive sensory neurons." dans: Nature communications, Vol. 9, Issue 1, pp. 1640, 2019 (PubMed).

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