DLG4 anticorps

N° du produit ABIN6658152

N° du produit (Fournisseur): 200-301-g32

Cet anticorps Souris Monoclonal détecte spécifiquement DLG4 dans WB, IHC, ELISA, IF et FM. Il présente une réactivité envers Rat.

Aperçu rapide pour DLG4 anticorps (ABIN6658152)

Antigène: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DLG4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Clone: 6G6

Détail du produit anti-DLG4 anticorps

N° du produit (Fournisseur): 200-301-g32

Fournisseur: Rockland

Fonction: PSD95 Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with PSD95 from mouse, rat, and bovine based on 100 % homology with the immunizing sequence.

Purification: Anti-PSD95 Antibody was purified by Protein G chromatography.

Stérilité: Sterile filtered

Immunogène: PSD95 Antibody was produced in mice by repeated immunizations raised against recombinant rat PSD-95.

Isotype: IgG2a

Information d'application

Indications d'application:

ELISA_Dilution: 1:250

Immunohistochemistry_Dilution: User Optimized

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: 1:250

Commentaires:

Anti-PSD95 Antibody is tested for WB, IHC, and IF microscopy. Expect a band approximately ~100kDa protein corresponding to the molecular mass of PSD-95 on SDS PAGE immunoblots. An additional protein of >100kDa is also detected. Additional cross-reactive bands are detected at ~75kDa and 50kDa in rat and mouse samples. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur DLG4

Antigène: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Autre désignation: PSD95

Sujet:

Synonyms: DLG4, SAP90, Synapse-associated protein 90, Postsynaptic density protein 95, Disks large homolog 4

Background: Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90 kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex. These family members consist of an N-terminal variable segment followed by three amino-terminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins. Thus different PDZ domains of PSD-95 might be specialized for distinct functions. PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning. Mutations in PSD95 are associated with autism.

Gene Name: Dlg4

ID gène: 29495

NCBI Accession: NP_031890

UniProt: P31016

Pathways: Regulation of Muscle Cell Differentiation, Synaptic Membrane, Skeletal Muscle Fiber Development, Asymmetric Protein Localization, Regulation of long-term Neuronal Synaptic Plasticity