RPH3A anticorps (pSer234)

N° du produit ABIN6658252

Détail du produit anti-RPH3A anticorps

Antigène: RPH3A (Rabphilin 3A (RPH3A))

Épitope: pSer234

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RPH3A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Réactivité croisée (Details): Cross reactivity with Rab 3A from other species has not been determined, however, the sequence of the immunogen shows 100 % identity to mouse, therefore, cross reactivity is expected.

Purification: Anti-Rabphilin 3A pS234 antibody is directed against rat rabphilin 3A protein phosphorylated at serine 234. Rabphilin 3A pS234 antibodies are affinity purified from monospecific antiserum by immunoaffinity purification. The immunolabeling of the rabphilin band was completely blocked by the phosphopeptide used as antigen while the dephosphopeptide had no effect on the immunolabeling.

Immunogène:

Immunogen: Anti-Rabphilin 3A pS234 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 conjugated to KLH.

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:400

Application Note: Anti-Rabphilin 3A pS234 (Rabbit) antibody is suitable for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 82 kDa in size corresponding to rabphilin 3A protein phosphorylated at Ser234 in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Détails sur RPH3A

Antigène: RPH3A (Rabphilin 3A (RPH3A))

Autre désignation: Rabphilin 3A (RPH3A Produits)

Synonymes: anticorps 2900002P20Rik, anticorps AU022689, anticorps AW108370, anticorps CG11556, anticorps Dmel\\CG11556, anticorps exophilin-1, anticorps fc61h10, anticorps rph3a, anticorps wu:fc61h10, anticorps rabphilin 3A, anticorps Rabphilin, anticorps rabphilin-3a, putative, anticorps rabphilin-3A, anticorps rabphilin 3A S homeolog, anticorps rabphilin 3A homolog (mouse), a, anticorps RPH3A, anticorps Rph3a, anticorps Rph, anticorps Smp_145120, anticorps Tsp_00335, anticorps rph3a.S, anticorps rph3aa

Sujet:

Synonyms: Rabphilin 3A Homolog, Rabphilin-3A, RP3A, Rph3A, Exophilin 1, Exophilin-1

Background: Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes. The expression of rabphilin 3A is reduced in an animal model of Huntington's disease. Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner. Anti-Rabphilin 3A antibody is ideal for investigators involved in Signal Transduction, Cell Signaling, and Neuroscience research.

Gene Name: RPH3A

ID gène: 171039

UniProt: P47709

Pathways: Synaptic Vesicle Exocytosis