BrdU anticorps (HRP)

N° du produit ABIN669255

Cet anticorps anti-BrdU est un anticorps Lapin Polyclonal détectant BrdU dans IHC (p), IHC (fro) et ELISA. Adapté pour .

Aperçu rapide pour BrdU anticorps (HRP) (ABIN669255)

Antigène: BrdU (Bromodeoxyuridine (BrdU))

Reactivité: Veuillez nous consulter SVP

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BrdU est conjugé à/à la HRP

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA

Détail du produit anti-BrdU anticorps (HRP)

Réactivité croisée: Humain, Rat

Réactivité croisée (Details): BrdU

Purification: Purified by Protein A.

Immunogène: BrdU conjugated to KLH

Isotype: IgG

Information d'application

Indications d'application: IHC-P 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur BrdU

Antigène: BrdU (Bromodeoxyuridine (BrdU))

Autre désignation: BrdU

Classe de substances: Chemical

Sujet:

Synonyms: Bromodeoxyuridine, Bromodeoxyuridine, 5-Bromo-2-deoxyuridine, 5-BrdU, proliferation Marker.

Background: The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis