APEX1 anticorps (AA 2-318) (DyLight 550)

N° du produit ABIN6719389

Cet anticorps Souris Monoclonal détecte spécifiquement APEX1 dans FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour APEX1 anticorps (AA 2-318) (DyLight 550) (ABIN6719389)

Antigène: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp APEX1 est conjugé à/à la DyLight 550

Application: Flow Cytometry (FACS)

Clone: 5C11

Détail du produit anti-APEX1 anticorps (DyLight 550)

Épitope: AA 2-318

Fonction: Anti-Human APE1 DyLight® 550 conjugated APEX1 Antibody(monoclonal, 5C11)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Human APE1 DyLight® 550 conjugated APEX1 Antibody (monoclonal, 5C11) (ABIN5692978)-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human APE1 recombinant protein (Position: P2-L318). Human APE1 shares 94% and 93% amino acid (aa) sequence identity with mouse and rat APE1, respectively.

Isotype: IgG2b

Information d'application

Indications d'application: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Akiyama, K., Seki, S., Oshida, T., Yoshida, M. C.Structure, promoter analysis and chromosomal assignment of the human APEX gene.Biochim. Biophys. Acta 1219: 15-25, 1994. 2. Chou, K.-M., Cheng, Y.-C.An exonucleolytic activity of human apurinic/apyrimidinic endonuclease on 3-prime mispaired DNA.Nature 415: 655-659, 2002. 3. Robson, C. N., Hochhauser, D., Craig, R., Rack, K., Buckle, V. J., Hickson, I. D.Structure of the human DNA repair gene HAP1 and its localisation to chromosome 14q11.2-12.Nucleic Acids Res. 20: 4417-4421, 1992.

Commentaires:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Détails sur APEX1

Antigène: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Autre désignation: APEX1

Sujet:

Synonyms: DNA- (apurinic or apyrimidinic site) lyase, APEX nuclease, APEN, Apurinic-apyrimidinic endonuclease 1, AP endonuclease 1, APE-1, REF-1, Redox factor-1, DNA- (apurinic or apyrimidinic site) lyase, mitochondrial, APEX1, APE, APE1, APEX, APX, HAP1, REF1

Background: APEX1, also called apurinic endonuclease (APE), is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime, 5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.

Poids moléculaire: 39 kDa

ID gène: 328

UniProt: P27695

Pathways: Réparation de l'ADN, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus