ANO1 anticorps

N° du produit ABIN6719430

Cet anticorps anti-ANO1 est un anticorps Polyclonal produit chez le Lapin, détectant ANO1 dans IHC, FACS et WB. Convient pour Humain.

Aperçu rapide pour ANO1 anticorps (ABIN6719430)

Antigène: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ANO1 est non-conjugé

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB)

Détail du produit anti-ANO1 anticorps

Fonction: Anti-TMEM16A/ANO1 Antibody

Séquence: VLMVELFMRE EQDKQQLLET WMEKERQKDE

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-TMEM16A/ANO1 Antibody (ABIN6719430). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human TMEM16A, which shares 83.8% amino acid (aa) sequence identity with both mouse and rat TMEM16A.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Commentaires:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur ANO1

Antigène: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Autre désignation: ANO1

Sujet:

Synonyms: Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1, Oral cancer overexpressed protein 2, Transmembrane protein 16A, Tumor-amplified and overexpressed sequence 2, ANO1, DOG1, ORAOV2, TAOS2, TMEM16A

Tissue Specificity: Broadly expressed with higher levels in liver, skeletal muscle and gastrointestinal muscles.

Background: Anoctamin-1 (ANO1), also known as oral cancer overexpressed 2 (ORAOV2) or tumor-amplified and overexpressed sequence 2 (TMEM16A), is a protein that in humans is encoded by the ANO1 gene. This gene belongs to a family of membrane proteins containing 8 transmembrane segments, and it is mapped to 11q13.3. ANO1 is a candidate calcium-activated chloride channel that mediates receptor-activated chloride currents in diverse physiologic processes, and it is thought to be responsible for a voltage-sensitive calcium-activated chloride current. Its overexpression was reported in esophageal squamous cell carcinoma and breast cancer progression Crofelemer, an antidiarrhoeal, inhibits this channel. ANO1 has eight transmembrane domains, its pore is large and non-selective, allowing other negatively charged species to permeate.

Poids moléculaire: 130 kDa

ID gène: 55107