RNF169 anticorps (AA 311-708)

N° du produit ABIN6719530

Cet anticorps Lapin Polyclonal détecte spécifiquement RNF169 dans WB, ELISA et IHC. Il présente une réactivité envers Humain, Rat et Souris.

Aperçu rapide pour RNF169 anticorps (AA 311-708) (ABIN6719530)

Antigène: RNF169 (Ring Finger Protein 169 (RNF169))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RNF169 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-RNF169 anticorps

Épitope: AA 311-708

Fonction: Anti-RNF169 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-R Antibody Picoband® (ABIN6719530). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human RNF169 recombinant protein (Position: N311-K708).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Chen J, Feng W, Jiang J, Deng Y, Huen MS. Ring finger protein RNF169 antagonizes the ubiquitin-dependent signaling cascade at sites of DNA damage. J Biol Chem. 2012 Aug 10,287(33):27715-22. doi: 10.1074/jbc.M112.373530. Epub 2012 Jun 25.

Commentaires:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur RNF169

Antigène: RNF169 (Ring Finger Protein 169 (RNF169))

Autre désignation: RNF169

Sujet:

Synonyms: E3 ubiquitin-protein ligase RNF169, RING finger protein 169, RING-type E3 ubiquitin transferase RNF169, RNF169, KIAA1991

Tissue Specificity: Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

Background: RING finger protein 169 (RNF169) is a probable E3 ubiquitin-protein ligase that acts as a negative regulator of the repair of double-strand breaks (DSBs) following DNA damage. RNF169 is recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSB repair. This gene is mapped to 11q13.4.

Poids moléculaire: 90 kDa

ID gène: 254225